Some enhancer is located in the upstream promoter, located in the downstream, so the effect of insulator
does not depend on the relative position of the insulator with promoter.
Therefore, the direction of the effect of insulator reason haven't really understood.
Has been found that there are two loci in trans activation way affect the function of the insulator.
Gene S2J (
Coding of nucleoprotein identify insulator, insulator and its combination insulation effect.
When the gene mutation, although y inserted into the insulator loci, but lost the insulation effect, y expressed in all tissues.
Another loci is mod (
, after the gene mutation, its effect as it happens and Su (
On the contrary, the type of these mutations are to enhance the insulation effect, make the insulation of the insulator effects no longer have directional and expanded, which is blocking the upstream and downstream sides to enhance the effect of the son.
One explanation is that first the Su (
After DNA combined with insulator, insulator have insulation effect.
With Su (
Combination, lose insulator insulation effect;
Mutation mod (
Not with Su (
Combination, so the insulator and to enhance the insulation effect.
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